1RNO
RIBONUCLEASE A CRYSTALLIZED FROM 80% AMMONIUM SULPHATE
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 290 |
| Detector technology | AREA DETECTOR |
| Collection date | 1992 |
| Detector | SIEMENS |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 65.100, 65.100, 65.520 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 8.000 - 1.900 |
| R-factor | 0.165 |
| Rwork | 0.165 |
| RMSD bond length | 0.015 * |
| RMSD bond angle | 3.090 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | X-PLOR |
| Refinement software | PROFFT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 8.400 | 1.990 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.041 | 0.177 * |
| Number of reflections | 12384 | |
| <I/σ(I)> | 25.62 | 0.177 |
| Completeness [%] | 94.9 | 84.1 |
| Redundancy | 2.72 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5.5 | pH 5.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 50 (mg/ml) | |
| 2 | 1 | reservoir | ammonium sulfate | 30 (%) | |
| 3 | 1 | reservoir | 3.0 (M) | ||
| 4 | 1 | reservoir | sodium acetate | 0.1 (M) |
