1RNA
CRYSTALLOGRAPHIC STRUCTURE OF AN RNA HELIX: [U(U-A)6A]2
Experimental procedure
| Temperature [K] | 291 |
| Detector technology | DIFFRACTOMETER |
| Detector | ENRAF-NONIUS CAD4 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 34.110, 44.610, 49.110 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.250 |
| R-factor | 0.131 |
| RMSD bond length | 0.040 * |
| RMSD bond angle | 0.035 |
| Refinement software | NUCLSQ |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 25.000 |
| High resolution limit [Å] | 2.250 |
| Number of reflections | 4924 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 35 * | pH 6.50, VAPOR DIFFUSION, HANGING DROP, temperature 308.00K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | WATER | ||
| 2 | 1 | 1 | MPD | ||
| 3 | 1 | 1 | NA CACODYLATE | ||
| 4 | 1 | 1 | SPERMINE | ||
| 5 | 1 | 1 | MG2+ | ||
| 6 | 1 | 2 | WATER | ||
| 7 | 1 | 2 | MPD |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | Methyl-2,2-pentanediol | 35 (%) | |
| 2 | 1 | reservoir | sodium cacodylate | 40 (mM) | |
| 3 | 1 | reservoir | magnesium | 400 (mM) | |
| 4 | 1 | reservoir | oligonucleotide | 4 (mM) |
