1RN8
Crystal structure of dUTPase complexed with substrate analogue imido-dUTP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-03-25 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.8110 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 74.618, 74.618, 99.580 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.930 |
| R-factor | 0.13906 |
| Rwork | 0.137 |
| R-free | 0.18017 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1euw |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.603 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.100 | 1.980 |
| High resolution limit [Å] | 1.930 | 1.930 |
| Number of reflections | 12768 | |
| <I/σ(I)> | 10.3 | 2.8 |
| Completeness [%] | 99.2 | 99.2 |
| Redundancy | 10 | 7.56 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 293 | PEG 3350, sodium acetate, Tris, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
