1RN1

THREE-DIMENSIONAL STRUCTURE OF GLN 25-RIBONUCLEASE T1 AT 1.84 ANGSTROMS RESOLUTION: STRUCTURAL VARIATIONS AT THE BASE RECOGNITION AND CATALYTIC SITES

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	91.710, 37.540, 77.670
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 1.840
R-factor	0.144
Rwork	0.144
RMSD bond length	0.018
RMSD bond angle	0.039 *
Phasing software	X-PLOR
Refinement software	PROLSQ

Data quality characteristics

	Overall
High resolution limit [Å]	1.840 *
Rmerge	0.046 *
Total number of observations	53463 *
Number of reflections	18062 *
Completeness [%]	75.1 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7 *	20 *	micro seeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir		55 (%)
2	1	reservoir	potasssium phosphate	0.1 (M)