1RMH

RECOMBINANT CYCLOPHILIN A FROM HUMAN T CELL

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	37.800, 109.200, 118.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 * - 2.400
R-factor	0.189
Rwork	0.189
R-free	0.25200 *
RMSD bond length	0.013
RMSD bond angle	3.100
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
High resolution limit [Å]	2.400 *	2.400 *
Rmerge	0.081 *
Total number of observations	50534 *
Number of reflections	16668 *
Completeness [%]	88.7 *	73.1 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	8.2 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	Tris-HCl	50 (mM)
10	1	reservoir	sodium azide	0.5 (mM)
2	1	drop	ammonium sulfate	3 (%sat)
3	1	drop	sodium azide	0.2 (mM)
4	1	drop	AAPE	0.8 (mM)
5	1	drop	CyPA	0.6 (mM)
6	1	drop	dimethyl sulfoxide	4 (%)
7	1	drop	glycerol	0.25 (%)
8	1	reservoir	Tris-HCl	100 (mM)
9	1	reservoir	ammonium sulfate	46 (%sat)