1RMH
RECOMBINANT CYCLOPHILIN A FROM HUMAN T CELL
Experimental procedure
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 37.800, 109.200, 118.700 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 * - 2.400 |
| R-factor | 0.189 |
| Rwork | 0.189 |
| R-free | 0.25200 * |
| RMSD bond length | 0.013 |
| RMSD bond angle | 3.100 |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| High resolution limit [Å] | 2.400 * | 2.400 * |
| Rmerge | 0.081 * | |
| Total number of observations | 50534 * | |
| Number of reflections | 16668 * | |
| Completeness [%] | 88.7 * | 73.1 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 8.2 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Tris-HCl | 50 (mM) | |
| 10 | 1 | reservoir | sodium azide | 0.5 (mM) | |
| 2 | 1 | drop | ammonium sulfate | 3 (%sat) | |
| 3 | 1 | drop | sodium azide | 0.2 (mM) | |
| 4 | 1 | drop | AAPE | 0.8 (mM) | |
| 5 | 1 | drop | CyPA | 0.6 (mM) | |
| 6 | 1 | drop | dimethyl sulfoxide | 4 (%) | |
| 7 | 1 | drop | glycerol | 0.25 (%) | |
| 8 | 1 | reservoir | Tris-HCl | 100 (mM) | |
| 9 | 1 | reservoir | ammonium sulfate | 46 (%sat) |
