1RMD
RAG1 DIMERIZATION DOMAIN
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE AREA DETECTOR |
| Collection date | 1996-06 |
| Detector | MARRESEARCH |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 57.580, 57.580, 98.430 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 2.100 |
| R-factor | 0.209 |
| Rwork | 0.209 |
| R-free | 0.25700 |
| Structure solution method | MAD |
| RMSD bond length | 0.010 |
| RMSD bond angle | 24.200 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | BAZFAZ |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.150 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.072 | 0.610 |
| Number of reflections | 36957 | |
| <I/σ(I)> | 24 | 3.1 |
| Completeness [%] | 93.9 | 95.6 |
| Redundancy | 3.6 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.5 | 5-10 % PEG 4K, 100MM HEPES, PH 7.5, 10% ETHYLENE GLYCOL |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG4000 | 5-10 (%) | |
| 2 | 1 | reservoir | HEPES | 100 (mM) | |
| 3 | 1 | reservoir | ethylene glycerol | 10 (%) |
