1RM0
Crystal Structure of Myo-Inositol 1-Phosphate Synthase From Saccharomyces cerevisiae In Complex With NAD+ and 2-deoxy-D-glucitol 6-(E)-vinylhomophosphonate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-ID |
| Synchrotron site | APS |
| Beamline | 17-ID |
| Detector technology | CCD |
| Collection date | 2002-08-09 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0093 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 151.810, 97.780, 122.290 |
| Unit cell angles | 90.00, 126.30, 90.00 |
Refinement procedure
| Resolution | 35.000 - 2.050 |
| R-factor | 0.189 |
| Rwork | 0.189 |
| R-free | 0.24400 |
| Structure solution method | FOURIER SYNTHESIS |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.800 | 2.090 |
| High resolution limit [Å] | 2.050 | 2.050 |
| Number of reflections | 110431 | |
| Completeness [%] | 98.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 298 | PEG 8000, SODIUM ACETATE, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
