1RLW
CALCIUM-PHOSPHOLIPID BINDING DOMAIN FROM CYTOSOLIC PHOSPHOLIPASE A2
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-07-07 |
| Detector | MARRESEARCH |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 79.410, 79.410, 70.670 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 15.000 - 2.400 |
| R-factor | 0.228 * |
| Rwork | 0.227 |
| R-free | 0.27300 |
| Structure solution method | MIR |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.025 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | SHARP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.000 | 2.350 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.086 | 0.310 |
| Total number of observations | 52266 * | |
| Number of reflections | 11798 | |
| <I/σ(I)> | 18 | 3.4 |
| Completeness [%] | 98.8 | 86 |
| Redundancy | 4.4 | 2.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 | 21 * | 5-10MG/ML PROTEIN, 5MM CACL2, 1M SODIUM ACETATE, 0.1M HEPES (PH 7.5), 50MM CDSO4 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 2.5-5 (mg/ml) | |
| 2 | 1 | drop | 2.5 (mM) | ||
| 3 | 1 | drop | sodium acetate | 0.5 (M) | |
| 4 | 1 | drop | HEPES | 0.05 (M) | |
| 5 | 1 | drop | 25 (mM) | ||
| 6 | 1 | reservoir | sodium acetate | 1 (M) | |
| 7 | 1 | reservoir | HEPES | 0.1 (M) | |
| 8 | 1 | reservoir | 50 (mM) |
