1RLU

Mycobacterium tuberculosis FtsZ in complex with GTP-gamma-S

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE A1
Synchrotron site	CHESS
Beamline	A1
Temperature [K]	100
Detector technology	CCD
Collection date	2000-07-29
Detector	ADSC QUANTUM 1
Spacegroup name	P 65
Unit cell lengths	88.413, 88.413, 178.522
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	14.940 - 2.080
R-factor	0.18206
Rwork	0.180
R-free	0.22442
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PARTIALLY REFINED MODEL OF THE MYCOBACTERIUM TUBERCULOSIS FTSZ/CITRATE COMPLEX PDB ENTRY 1RQ2.
RMSD bond length	0.020
RMSD bond angle	1.681
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	EPMR
Refinement software	REFMAC (5.1.27)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	2.150
High resolution limit [Å]	2.080	2.080
Number of reflections	44782
<I/σ(I)>	9.7	1.7
Completeness [%]	94.6	50.7
Redundancy	4.3	1.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		5.6	293	30% PEG 4000, 0.1M sodium citrate, 0.2M ammonium acetate, 2MM SRI-7614, ETHYL (6-AMINO-2,3-DIHYDRO-4-PHENYL-1H-PYRIDO[4,3-B][1,4]DIAZEPIN-8-YL)-CARBAMATE, WAS INCLUDED AS WELL, BUT WAS NOT LOCATED IN THE FINAL STRUCTURE, VAPOR DIFFUSION, SITTING DROP, temperature 293K, pH 5.60