1RLT
Transition State Analogue of ybiV from E. coli K12
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-07-12 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 1.000 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 71.761, 91.166, 183.850 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.930 - 2.200 |
R-factor | 0.221 |
Rwork | 0.221 |
R-free | 0.26400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ID 1RLM |
RMSD bond length | 0.009 |
RMSD bond angle | 1.800 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS (1.1) |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.240 |
High resolution limit [Å] | 2.200 | 2.200 |
Number of reflections | 61606 | |
<I/σ(I)> | 31 | 5 |
Completeness [%] | 99.7 | 100 |
Redundancy | 5.4 | 5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.3 | 298 | PEG 8000, sodium acetate, aluminum chloride, sodium fluoride, cacodylic acid, magnesium chloride, pH 5.3, VAPOR DIFFUSION, HANGING DROP, temperature 298K |