1RLS
CRYSTAL STRUCTURE OF RNASE T1 COMPLEXED WITH THE PRODUCT NUCLEOTIDE 3'-GMP. STRUCTURAL EVIDENCE FOR DIRECT INTERACTION OF HISTIDINE 40 AND GLUTAMIC ACID 58 WITH THE 2'-HYDROXYL GROUP OF RIBOSE
Experimental procedure
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 48.140, 51.060, 40.380 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.900 |
| R-factor | 0.167 |
| RMSD bond length | 0.017 |
| RMSD bond angle | 0.040 |
| Phasing software | X-PLOR |
| Refinement software | PROFFT |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 4 * | 15 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | enzyme | 1.0 (mM) | |
| 2 | 1 | reservoir | MPD | 60 (%(v/v)) | |
| 3 | 1 | reservoir | acetate | 10 (mM) | |
| 4 | 1 | drop | 3'-GMP | 1.0 (mM) |
