1RLK
Structure of Conserved Protein of Unknown Function TA0108 from Thermoplasma acidophilum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-08-10 |
| Detector | SBC-2 |
| Wavelength(s) | 0.97915 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 43.456, 43.456, 103.381 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.530 - 1.950 |
| R-factor | 0.21621 |
| Rwork | 0.212 |
| R-free | 0.23894 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.154 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.990 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.058 | 0.405 |
| Number of reflections | 8692 | |
| <I/σ(I)> | 8.9 | |
| Completeness [%] | 99.0 | 98.3 |
| Redundancy | 16.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 294 | Calcium chloride, PEG 8000, glycerol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
