1RLC

CRYSTAL STRUCTURE OF THE UNACTIVATED RIBULOSE 1, 5-BISPHOSPHATE CARBOXYLASE(SLASH)OXYGENASE COMPLEXED WITH A TRANSITION STATE ANALOG, 2-CARBOXY-D-ARABINITOL 1,5-BISPHOSPHATE

Experimental procedure

Spacegroup name	I 4 2 2
Unit cell lengths	149.000, 149.000, 136.800
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	? - 2.700
R-factor	0.196
Rwork	0.196
RMSD bond length	0.018
RMSD bond angle	3.630
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.700 *
Rmerge	0.092 *
Total number of observations	69151 *
Number of reflections	19091 *
Completeness [%]	92.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir		0.2 (M)
2	1	reservoir	ammonium sulfate	0.3 (M)
3	1	reservoir		1 (mM)
4	1	drop	rubisco	30-70 (%(v/v))
5	1	drop		0.2 (M)
6	1	drop	ammonium sulfate	0.3 (M)
7	1	drop		1 (mM)