1RLB

RETINOL BINDING PROTEIN COMPLEXED WITH TRANSTHYRETIN

Experimental procedure

Detector technology	IMAGE PLATE
Detector	RIGAKU
Wavelength(s)	1.5418
Spacegroup name	I 2 2 2
Unit cell lengths	222.400, 163.400, 55.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 3.100
R-factor	0.215
Rwork	0.215
RMSD bond length	0.018
RMSD bond angle	26.700 *
Data reduction software	MSC
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	6.000 *
High resolution limit [Å]	3.100 *	3.100 *
Rmerge	0.090
Total number of observations	47491 *
Number of reflections	17465	2359 *
Completeness [%]	94.8	83.2 *
Redundancy	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Microdialysis *	5.5 *	4 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	15-20 (mg/ml)
2	1	2	succinate	0.1 (M)
3	1	2	ethylene glycol	3 (%)
4	1	2	ammonium sulfate	2.3 (M)