1RL3

Crystal structure of cAMP-free R1a subunit of PKA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	200
Detector technology	CCD
Collection date	2002-12-17
Detector	ADSC QUANTUM 4
Wavelength(s)	1.08
Spacegroup name	P 65
Unit cell lengths	90.300, 90.300, 177.600
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	50.000 - 2.700
Rwork	0.240
R-free	0.28500
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	1rgs
RMSD bond length	0.009
RMSD bond angle	1.700
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.800
High resolution limit [Å]	2.700	2.700
Rmerge	0.053	0.458
Number of reflections	21075
<I/σ(I)>		2.5
Completeness [%]	93.7	93.1
Redundancy	2.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	295.5	NH4SO4, glycerol, DTT, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295.5K