1RL0

Crystal structure of a new ribosome-inactivating protein (RIP): dianthin 30

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	CCD
Collection date	2002-12-07
Detector	ADSC QUANTUM 210
Spacegroup name	P 1 21 1
Unit cell lengths	37.568, 78.654, 43.136
Unit cell angles	90.00, 105.39, 90.00

Refinement procedure

Resolution	36.220 - 1.400
R-factor	0.1841
Rwork	0.184
R-free	0.20600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PBD ENTRY 1QI7
RMSD bond length	0.008
RMSD bond angle	1.200
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.000	1.450
High resolution limit [Å]	1.400	1.400
Number of reflections	44547
<I/σ(I)>	26.3	10.4
Completeness [%]	93.8	90.6
Redundancy	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		4.5	293	PEG 4000, ammonium acetate, sodium acetate, pH 4.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K, pH 4.50