1RKU
Crystal Structure of ThrH gene product of Pseudomonas Aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-10-20 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97679 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 71.749, 97.236, 131.772 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.470 |
| R-factor | 0.1906 |
| Rwork | 0.189 |
| R-free | 0.22092 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.528 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.1.27) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.520 |
| High resolution limit [Å] | 1.470 | 1.470 |
| Number of reflections | 74797 | |
| <I/σ(I)> | 48.36 | 2.5 |
| Completeness [%] | 96.0 | 71.9 |
| Redundancy | 18 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | PEG 1000, Sodium cacodylate, MGCL2, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
