1RKB
The structure of adrenal gland protein AD-004
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR591 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-03-03 |
| Detector | BRUKER SMART 6000 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 61 |
| Unit cell lengths | 99.555, 99.555, 57.191 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.780 - 2.000 |
| R-factor | 0.203 |
| Rwork | 0.203 |
| R-free | 0.21800 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.200 |
| Data reduction software | PROTEUM PLUS |
| Data scaling software | PROTEUM PLUS |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 57.000 | 2.040 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 21969 | |
| <I/σ(I)> | 15.15 | 1.73 |
| Completeness [%] | 99.7 | 99.38 |
| Redundancy | 5.15 | 2.37 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | HEPES-Na, Lithium Sulfate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
