1RKA

THE APO FORM OF E. COLI RIBOKINASE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MAX II BEAMLINE I711
Synchrotron site	MAX II
Beamline	I711
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1998-05-15
Detector	MARRESEARCH
Spacegroup name	P 31 2 1
Unit cell lengths	61.970, 61.970, 127.834
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	15.000 - 2.300
Rwork	0.210
R-free	0.25300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1RKD: RESIDUES 4-9 44-93 123-241 AND 254-309.
RMSD bond length	0.006 *
RMSD bond angle	1.450 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (0.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	2.340
High resolution limit [Å]	2.300	2.300
Rmerge	0.056	0.235
Total number of observations	62741 *
Number of reflections	12644
<I/σ(I)>	20.6	6.7
Completeness [%]	96.4	87.5
Redundancy	5.5	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	4.8		drop consists of equal volume of protein and reservoir solutions *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	ribokinase	11 (mg/ml)
2	1	drop	Tris-HCl	20 (mM)
3	1	drop		0.2 (M)
4	1	reservoir	Na acetate	0.1 (M)
5	1	reservoir	NH4 acetate	0.2 (M)
6	1	reservoir	mPEG2000	22-33 (%)