1RJO
AGAO + Xe
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-05-28 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.54 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 158.009, 63.189, 92.149 |
| Unit cell angles | 90.00, 112.09, 90.00 |
Refinement procedure
| Resolution | 26.300 - 1.670 |
| R-factor | 0.15843 |
| Rwork | 0.157 |
| R-free | 0.17804 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | AGAO |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.139 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 26.300 | 1.730 |
| High resolution limit [Å] | 1.670 | 1.670 |
| Rmerge | 0.050 | 0.288 |
| Number of reflections | 94497 | |
| <I/σ(I)> | 13.9 | 3.54 |
| Completeness [%] | 97.1 | 93.6 |
| Redundancy | 3 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | DROP: 0.0015ml of protein solution (11.7mg/ml, 50mM HEPES pH 7.0) plus 0.0015ml of reservoir solution (1.0-1.5M NH4SO4, 2mM CUSO4, 150mM sodium citrate pH 6.0-7.5), VAPOR DIFFUSION, HANGING DROP, temperature 293K |
