1RJC

Crystal structure of the camelid single domain antibody cAb-Lys2 in complex with hen egg white lysozyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7B
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7B
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2002-10-28
Detector	MARRESEARCH
Wavelength(s)	0.8453
Spacegroup name	P 21 21 21
Unit cell lengths	50.020, 68.360, 72.050
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	26.880 - 1.400
R-factor	0.22
Rwork	0.220
R-free	0.23500
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.400
Data reduction software	DENZO
Data scaling software	CCP4 ((TRUNCATE))
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.400	1.450
High resolution limit [Å]	1.400	1.400
Rmerge	0.065	0.371
Number of reflections	48909
<I/σ(I)>	21.4	3.7
Completeness [%]	99.1	96.8
Redundancy	4.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	298	PEG 8000, glycerol, ammonium sulphate, potassium phosphate, sodium chloride, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K