1RJ2
Crystal structure of the DH/PH fragment of Dbs without bound GTPase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-02-22 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 97.989, 82.824, 127.257 |
| Unit cell angles | 90.00, 90.80, 90.00 |
Refinement procedure
| Resolution | 30.000 - 3.000 |
| Rwork | 0.239 |
| R-free | 0.29570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1KZ7 (DH and PH domains) |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.267 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | BEAST |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.110 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Number of reflections | 40298 | |
| <I/σ(I)> | 13.3 | 1.5 |
| Completeness [%] | 96.9 | 88.7 |
| Redundancy | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | Na HEPES, PEG 20K, ethylene glycol, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
