1RIY
HU mutant V42I from Thermotoga maritima
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-08-16 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 1.0727 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 45.447, 45.447, 76.204 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.870 - 1.800 |
| R-factor | 0.21922 |
| Rwork | 0.216 |
| R-free | 0.28590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1b8z |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.355 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.038 | 0.481 |
| Number of reflections | 7873 | |
| <I/σ(I)> | 16.9 | 3.9 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 7.5 | 7.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 4.3 | 293 | 100mM Na-acetate, pH 4.0-4.5, 80-90% saturated ammonium sulphate, pH 4.3, VAPOR DIFFUSION, temperature 293K |
