1RIQ
The crystal structure of the catalytic fragment of the alanyl-tRNA synthetase
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2003-03-03 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9793, 0.9795, 1.0332 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 74.890, 74.890, 179.950 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.140 |
| R-factor | 0.2069 |
| Rwork | 0.207 |
| R-free | 0.25140 |
| Structure solution method | MAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.270 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.190 |
| High resolution limit [Å] | 2.100 | 2.110 |
| Rmerge | 0.081 | 0.382 |
| Number of reflections | 26377 | |
| <I/σ(I)> | 13.6 | 13.1 |
| Completeness [%] | 93.6 | 89.1 |
| Redundancy | 3.5 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.2 | 298 | PEG 5000mme, NaCl, Tris, pH 6.2, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
