1RIO
Structure of bacteriophage lambda cI-NTD in complex with sigma-region4 of Thermus aquaticus bound to DNA
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X9A |
| Synchrotron site | NSLS |
| Beamline | X9A |
| Detector technology | CCD |
| Collection date | 2002-10-15 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97938,0.97927,0.9648 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.256, 71.269, 77.199 |
| Unit cell angles | 90.00, 91.34, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.300 |
| R-factor | 0.218 |
| Rwork | 0.216 |
| R-free | 0.25400 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 * |
| RMSD bond angle | 1.330 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 2.250 |
| Number of reflections | 22889 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.5 * | 22 * | MPD, Sodium Acetate, calcium chloride, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 295K, pH 4.60 |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | MPD | ||
| 2 | 1 | 1 | Sodium Acetate | ||
| 3 | 1 | 1 | calcium chloride | ||
| 4 | 1 | 1 | H2O | ||
| 5 | 1 | 2 | Sodium Acetate | ||
| 6 | 1 | 2 | calcium chloride | ||
| 7 | 1 | 2 | H2O |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | 18 (%) | ||
| 2 | 1 | reservoir | 20 (mM) | ||
| 3 | 1 | reservoir | sodium acetate | 0.1 (M) | pH4.6 |
| 4 | 1 | drop | protein | 25 (mg/ml) | |
| 5 | 1 | drop | HEPES | 10 (mM) | pH7.5 |
| 6 | 1 | drop | 150 (mM) | ||
| 7 | 1 | drop | EDTA | 0.1 (mM) |
