1RIL

CRYSTAL STRUCTURE OF RIBONUCLEASE H FROM THERMUS THERMOPHILUS HB8 REFINED AT 2.8 ANGSTROMS RESOLUTION

Experimental procedure

Spacegroup name	P 65 2 2
Unit cell lengths	44.700, 44.700, 314.700
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	8.000 - 2.800
R-factor	0.205
RMSD bond length	0.019
RMSD bond angle	0.048
Refinement software	PROLSQ

Data quality characteristics

	Overall
High resolution limit [Å]	2.800 *
Rmerge	0.093 *
Total number of observations	46156 *
Number of reflections	4766 *
Completeness [%]	89.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	9.8 *		taken from Okumura, M. et al (1993). Proteins Struct. Funct. Genet., 15, 108-111. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	Tris-HCl	18 (mM)
2	1	drop	dithiothreitol	5 (mM)
3	1	reservoir	Tris-HCl	50 (mM)