1RII
Crystal structure of phosphoglycerate mutase from M. Tuberculosis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-07-11 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 58.914, 136.790, 65.929 |
| Unit cell angles | 90.00, 97.78, 90.00 |
Refinement procedure
| Resolution | 58.370 - 1.700 |
| R-factor | 0.2193 |
| R-free | 0.26970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5pgm |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.022 |
| Data reduction software | DENZO |
| Data scaling software | SADABS (V. 2003 (SHELDRICK)) |
| Phasing software | GLRF |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 58.370 | 1.800 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 111293 | |
| <I/σ(I)> | 18.1 | 3.2 |
| Completeness [%] | 98.2 | 91.8 |
| Redundancy | 6.5 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 298 | 15% PEG 3350, 0.1M magnesium chloride, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 298K, pH 7.00 |
