1RIF
Crystal structure of the UvsW helicase from Bacteriophage T4
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Wavelength(s) | 1.0383, 1.03900 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.739, 95.181, 86.278 |
| Unit cell angles | 90.00, 95.40, 90.00 |
Refinement procedure
| Resolution | 30.000 * - 2.000 |
| R-factor | 0.221 |
| Rwork | 0.218 |
| R-free | 0.24600 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 * |
| RMSD bond angle | 1.340 * |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.036 | |
| Number of reflections | 42780 | |
| <I/σ(I)> | 16.9 | |
| Completeness [%] | 92.1 | 74.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 18 * | Amonium sulfate, magnesium chloride, Tris-HCl, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | reservoir | PEG3350 | 25 (%) | |
| 3 | 1 | reservoir | Tris-HCl | 100 (mM) | pH7.5 |
| 4 | 1 | reservoir | 200 (mM) |
