1RIE
STRUCTURE OF A WATER SOLUBLE FRAGMENT OF THE RIESKE IRON-SULFUR PROTEIN OF THE BOVINE HEART MITOCHONDRIAL CYTOCHROME BC1-COMPLEX
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-05-10 |
| Detector | FUJI |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 32.100, 53.000, 38.000 |
| Unit cell angles | 90.00, 100.30, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.500 |
| R-factor | 0.187 |
| Rwork | 0.187 |
| R-free | 0.21100 |
| Structure solution method | MAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 27.000 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.051 | 0.270 |
| Total number of observations | 63633 * | |
| Number of reflections | 18058 | |
| <I/σ(I)> | 28.6 | 5.5 |
| Completeness [%] | 89.4 | 68 |
| Redundancy | 3.52 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.2 * | 22 * | pH 6.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 2.5-3 (mg/ml) | |
| 2 | 1 | drop | 2 (mM) | ||
| 3 | 1 | reservoir | PEG6000 | 20-30 (%) | |
| 4 | 1 | reservoir | MES | 100 (mM) |
