1RIE

STRUCTURE OF A WATER SOLUBLE FRAGMENT OF THE RIESKE IRON-SULFUR PROTEIN OF THE BOVINE HEART MITOCHONDRIAL CYTOCHROME BC1-COMPLEX

Experimental procedure

Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6A
Synchrotron site	Photon Factory
Beamline	BL-6A
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1995-05-10
Detector	FUJI
Spacegroup name	P 1 21 1
Unit cell lengths	32.100, 53.000, 38.000
Unit cell angles	90.00, 100.30, 90.00

Refinement procedure

Resolution	10.000 - 1.500
R-factor	0.187
Rwork	0.187
R-free	0.21100
Structure solution method	MAD
RMSD bond length	0.009
RMSD bond angle	27.000 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MLPHARE
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	1.530
High resolution limit [Å]	1.500	1.500
Rmerge	0.051	0.270
Total number of observations	63633 *
Number of reflections	18058
<I/σ(I)>	28.6	5.5
Completeness [%]	89.4	68
Redundancy	3.52

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.2 *	22 *	pH 6.0

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	2.5-3 (mg/ml)
2	1	drop		2 (mM)
3	1	reservoir	PEG6000	20-30 (%)
4	1	reservoir	MES	100 (mM)