1RI7

crystal structure of a protein in the LRP/ASNC family from the hyperthermophilic archaeon Pyrococcus sp. OT3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-18B
Synchrotron site	Photon Factory
Beamline	BL-18B
Temperature [K]	100
Detector technology	CCD
Collection date	2002-01-20
Detector	ADSC QUANTUM 4
Wavelength(s)	1.0
Spacegroup name	P 61 2 2
Unit cell lengths	130.910, 130.910, 47.370
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.000 - 2.700
R-factor	0.246
Rwork	0.246
R-free	0.31400
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	22.700 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.000	2.850
High resolution limit [Å]	2.700	2.700
Rmerge	0.059	0.261
Number of reflections	6914
<I/σ(I)>	11	2.9
Completeness [%]	99.3	98
Redundancy	11.7	11.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8 *	20 *	ammonium sulfate, tris[hydroxymethyl]aminomethan, glycerol, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	drop	sodium phosphate	50 (mM)	pH8.0
3	1	drop		300 (mM)
4	1	reservoir	Tris-HCl	0.1 (M)	pH8.5
5	1	reservoir	ammonium sulfate	1.5 (M)
6	1	reservoir	glycerol	12 (%(v/v))