1RI7
crystal structure of a protein in the LRP/ASNC family from the hyperthermophilic archaeon Pyrococcus sp. OT3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-18B |
| Synchrotron site | Photon Factory |
| Beamline | BL-18B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-01-20 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 130.910, 130.910, 47.370 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.000 - 2.700 |
| R-factor | 0.246 |
| Rwork | 0.246 |
| R-free | 0.31400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 22.700 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.000 | 2.850 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.059 | 0.261 |
| Number of reflections | 6914 | |
| <I/σ(I)> | 11 | 2.9 |
| Completeness [%] | 99.3 | 98 |
| Redundancy | 11.7 | 11.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 * | 20 * | ammonium sulfate, tris[hydroxymethyl]aminomethan, glycerol, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | drop | sodium phosphate | 50 (mM) | pH8.0 |
| 3 | 1 | drop | 300 (mM) | ||
| 4 | 1 | reservoir | Tris-HCl | 0.1 (M) | pH8.5 |
| 5 | 1 | reservoir | ammonium sulfate | 1.5 (M) | |
| 6 | 1 | reservoir | glycerol | 12 (%(v/v)) |
