1RHP

CRYSTAL STRUCTURE OF RECOMBINANT HUMAN PLATELET FACTOR 4

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	78.200, 86.200, 43.400
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.400
R-factor	0.241
Rwork	0.241
RMSD bond length	0.016
RMSD bond angle	3.890
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	8.000 *
High resolution limit [Å]	2.400 *	2.400 *
Rmerge	0.060 *
Number of reflections	11037 *	1202 *
Completeness [%]	94.4 *	81.5 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5 *	20 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	12 (mg/ml)
2	1	drop	sodium acetate	10 (mM)
3	1	drop	sodium chloride	150 (mM)
4	1	reservoir			pH is adjusted to 7.9 by NH4OH
5	1	reservoir	sodium acetate	10 (mM)