1RHG

THE STRUCTURE OF GRANULOCYTE-COLONY-STIMULATING FACTOR AND ITS RELATIONSHIP TO THOSE OF OTHER GROWTH FACTORS

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	90.900, 110.700, 49.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 * - 2.200
R-factor	0.215
Rwork	0.215
R-free	0.34400 *
RMSD bond length	0.017
RMSD bond angle	3.354
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.200 *
Rmerge	0.056 *
Total number of observations	50868 *
Number of reflections	10735 *
Completeness [%]	93.3 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5.8 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG8000	8 (%(w/v))
2	1	reservoir		380 (mM)
3	1	reservoir		220 (mM)