1RH9
Family GH5 endo-beta-mannanase from Lycopersicon esculentum (tomato)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-03-01 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.900 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 59.540, 74.521, 79.006 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.500 |
| R-factor | 0.18364 |
| Rwork | 0.182 |
| R-free | 0.20479 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qnp |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.224 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.1.19) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.233 | 1.550 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.080 | 0.743 |
| Number of reflections | 56178 | |
| <I/σ(I)> | 12.1 | 1.2 |
| Completeness [%] | 98.4 | 99.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 298 | 30% PEG 4000, 0.1M Na Citrate, 0.2M NH4 Acetate, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
