1RGV
Crystal Structure of the Ferredoxin from Thauera aromatica
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 295 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-02-18 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 79.000, 79.000, 49.300 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.860 - 2.900 |
| R-factor | 0.197 |
| Rwork | 0.197 |
| R-free | 0.21900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Ferredoxin from Allochromatium vinosum (PDB 1BLU) |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.220 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.000 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.081 * | 0.370 * |
| Number of reflections | 3753 * | |
| <I/σ(I)> | 12.4 | 2.7 |
| Completeness [%] | 95.7 | 70 |
| Redundancy | 2.9 * | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 * | 303 * | sodium citrate, ammonium phosphate, pH 5.8, VAPOR DIFFUSION, HANGING DROP, temperature 281K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG4000 | 10 (%(w/v)) | |
| 2 | 1 | reservoir | MPD | 15 (%(w/v)) | |
| 3 | 1 | reservoir | HEPES | 0.1 (M) | pH7.5 |
| 4 | 1 | drop | protein | 15-20 (mg/ml) | |
| 5 | 1 | drop | triethanolamine | 5 (mM) | |
| 6 | 1 | drop | 2 (mM) | ||
| 7 | 1 | drop | dithiothreitol | 2 (mM) | pH7.8 |
