1RGS

REGULATORY SUBUNIT OF CAMP DEPENDENT PROTEIN KINASE

Experimental procedure

Detector technology	AREA DETECTOR
Collection date	1993
Detector	XUONG-HAMLIN MULTIWIRE
Spacegroup name	P 65 2 2
Unit cell lengths	88.900, 88.900, 179.900
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	8.000 - 2.800
R-factor	0.217
Rwork	0.217
RMSD bond length	0.019
RMSD bond angle	2.800
Data reduction software	UCSD
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	2.800 *
Rmerge	0.055 *
Number of reflections	16785 *
Completeness [%]	94.0 *
Redundancy	3.6 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5.5 *	22 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	8 (mg/ml)
2	1	drop	ammonium sulfate	0.55-0.6 (M)
3	1	drop	glycerol	5-6.25 (%)
4	1	drop	dithiothreitol	5 (mM)
5	1	drop	sodium acetate	40-50 (mM)
6	1	reservoir	ammonium sulfate	1.1-1.2 (M)
7	1	reservoir	glycerol	10-12.5 (%)
8	1	reservoir	dithiothreitol	10 (mM)
9	1	reservoir	sodium acetate	80-100 (mM)