1RGP

GTPASE-ACTIVATION DOMAIN FROM RHOGAP

Experimental procedure

Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X31
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X31
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1996-10
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	36.086, 70.086, 79.205
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.000
R-factor	0.22 *
Rwork	0.220
R-free	0.28300
Structure solution method	MIR/MAD
RMSD bond length	0.016 *
RMSD bond angle	1.800 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CCP4
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.100
High resolution limit [Å]	2.000	2.000
Rmerge	0.097	0.380
Number of reflections	14248
<I/σ(I)>	10.3	2.1
Completeness [%]	99.7	95
Redundancy	3.5	3.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7.5	18 *	10% PEG 6K, 5% MPD IN 0.1M HEPES PH 7.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG6000	10 (%)
2	1	reservoir	MPD	5 (%)
3	1	reservoir	HEPES	0.1 (M)