1RGK

RNASE T1 MUTANT GLU46GLN BINDS THE INHIBITORS 2'GMP AND 2'AMP AT THE 3' SUBSITE

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	49.100, 46.690, 41.110
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	? - 1.870
R-factor	0.142
RMSD bond length	0.009
RMSD bond angle	1.784
Refinement software	TNT

Data quality characteristics

	Overall
Low resolution limit [Å]	10.000 *
High resolution limit [Å]	1.870 *
Rmerge	0.036 *
Total number of observations	11554 *
Number of reflections	6822 *
Completeness [%]	83.3 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Microdialysis *	4.2 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	E46Q	1.3 (mM)
2	1	1	2'AMP	14.5 (mM)
3	1	2	2'AMP	1 (mM)
4	1	2	sodium acetate	20 (mM)
5	1	2	calcium acetate	2 (mM)