1RGC
THE COMPLEX BETWEEN RIBONUCLEASE T1 AND 3'-GUANYLIC ACID SUGGESTS GEOMETRY OF ENZYMATIC REACTION PATH. AN X-RAY STUDY
Experimental procedure
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.680, 48.330, 40.480 |
| Unit cell angles | 90.00, 90.62, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.000 |
| R-factor | 0.153 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.900 |
| Refinement software | TNT |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 2.200 * |
| High resolution limit [Å] | 2.000 * |
| Completeness [%] | 76.0 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 4 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Ribonuclease T1 | 1 (%) | |
| 2 | 1 | drop | Gp[S]U | 1 (mM) | |
| 3 | 1 | reservoir | MPD | 50 (%) |
