1RGA

CRYSTAL STRUCTURE OF RNASE T1 WITH 3'-GMP AND GUANOSINE: A PRODUCT COMPLEX

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	47.300, 50.700, 40.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 1.700
R-factor	0.145
RMSD bond length	0.021
RMSD bond angle	0.059
Refinement software	PROLSQ

Data quality characteristics

	Overall
Low resolution limit [Å]	10.000 *
High resolution limit [Å]	1.700 *
Rmerge	0.067 *
Total number of observations	31376 *
Number of reflections	10878 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	4.2 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	RNaseT1	20 (mg/ml)
2	1	drop	exo cGPS	15 (mM)
3	1	drop	sodium acetate	20 (mM)
4	1	drop	calcium acetate	2 (mM)
5	1	reservoir	MPD	53 (%(v/v))