1RG9
S-Adenosylmethionine synthetase complexed with SAM and PPNP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 93 |
| Detector technology | IMAGE PLATE |
| Collection date | 2003-01-01 |
| Detector | RIGAKU RAXIS IIC |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 225.820, 69.130, 118.230 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.500 |
| R-factor | 0.212 |
| Rwork | 0.211 |
| R-free | 0.24200 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 27.500 * |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.093 * | 0.187 * |
| Total number of observations | 165302 * | |
| Number of reflections | 46081 | |
| Completeness [%] | 95.0 | 78 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 23 * | PEG-8000, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 296K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG8000 | 8 (%(v/w)) | |
| 2 | 1 | reservoir | Tris-HCl | 50 (mM) | pH8.0 |
| 3 | 1 | reservoir | 200 (mM) | ||
| 4 | 1 | reservoir | dithiothreitol | 1 (mM) | |
| 5 | 1 | reservoir | ethylene glycol | 20 (%) | |
| 6 | 1 | drop | HEPES-K+ | 50 (mM) | |
| 7 | 1 | drop | protein | 20 (mg/ml) |
