1RG5
Structure of the photosynthetic reaction centre from Rhodobacter sphaeroides carotenoidless strain R-26.1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2002-02-07 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.87 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 144.033, 144.033, 190.072 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 23.340 - 2.500 |
| R-factor | 0.15672 |
| Rwork | 0.155 |
| R-free | 0.18188 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.686 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.1.9999) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.340 | 2.540 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.074 | 0.864 |
| Number of reflections | 78686 | |
| <I/σ(I)> | 11 | 2.2 |
| Completeness [%] | 99.1 | 99 |
| Redundancy | 4.7 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 289 | Trisodium citrate, LDAO, 1,2,3-heptanetriol, Tris-HCl, pH 8.0, VAPOR DIFFUSION, SITTING DROP, temperature 16.0K |
