1RFP
ANALYSIS OF THE STABILIZATION OF HEN LYSOZYME WITH THE HELIX DIPOLE AND CHARGED SIDE CHAINS
Experimental procedure
| Temperature [K] | 295 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-11-20 |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 79.210, 79.210, 37.970 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 6.000 - 1.750 |
| R-factor | 0.168 |
| Rwork | 0.168 |
| Starting model (for MR) | 1hel |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.448 |
| Data reduction software | R-AXIS (SOFTWARE) |
| Data scaling software | R-AXIS |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 1.800 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.037 | 0.191 |
| Number of reflections | 11948 | |
| Completeness [%] | 93.2 | 78.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 4.7 | 50 MM ACETATE AT PH 4.7 CONTAINING 0.9 M NACL |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | sodium acetate | 50 (mM) | |
| 2 | 1 | reservoir | 0.9-1.2 (M) |
