1RFN

HUMAN COAGULATION FACTOR IXA IN COMPLEX WITH P-AMINO BENZAMIDINE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Temperature [K]	280
Spacegroup name	P 61 2 2
Unit cell lengths	90.900, 90.900, 151.600
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	25.000 - 2.800
R-factor	0.216 *
Rwork	0.216
R-free	0.27300
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	2.000
Refinement software	X-PLOR (3.8)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.800	2.800 *
Rmerge	0.077	0.316 *
Number of reflections	16348
Completeness [%]	92.7	82 *
Redundancy	2.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	8	4 *	pH 8.00

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	PAPB	2 (mM)
2	1	reservoir	Tris	100 (mM)
3	1	reservoir	t-butanol	25 (%)
4	1	reservoir		10 (mM)
5	1	drop	protain	10 (mg/ml)