1RFK

Crystal Structure of 2Fe2S Ferredoxin from Thermophilic Cyanobacterium Mastigocladus Laminosus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-1
Synchrotron site	ESRF
Beamline	ID14-1
Temperature [K]	100
Detector technology	CCD
Collection date	2002-06-17
Detector	ADSC QUANTUM 4
Wavelength(s)	0.933
Spacegroup name	P 21 21 21
Unit cell lengths	28.450, 50.934, 110.913
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.000 - 1.250
R-factor	0.211
Rwork	0.199
R-free	0.24700
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	1.270
High resolution limit [Å]	1.250	1.250
Number of reflections	44557
<I/σ(I)>	18.9
Completeness [%]	98.0	96
Redundancy	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	298	40% PEG 4000, 0.3M Mg(NO3)2, 20mM Tris-HCl, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K