1REM
HUMAN LYSOZYME WITH MAN-B1,4-GLCNAC COVALENTLY ATTACHED TO ASP53
Experimental procedure
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS IIC |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 36.390, 116.380, 30.910 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.100 |
| R-factor | 0.168 |
| Rwork | 0.168 |
| R-free | 0.24800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1REY EXCEPT RESIDUE NAG 131 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 24.225 * |
| Data reduction software | R-AXIS (SOFTWARE 2.1) |
| Data scaling software | R-AXIS (IIC V. 2.1) |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 36.400 |
| High resolution limit [Å] | 2.070 |
| Rmerge | 0.057 |
| Total number of observations | 22724 * |
| Number of reflections | 7438 |
| Completeness [%] | 94.9 |
| Redundancy | 3.05 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 4.5 | Muraki, M., (1991) Biochim. Biophys. Acta 1079, 229. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | sodium acetate | 25 (mM) | |
| 2 | 1 | drop | ammonium nitrate | 3 (M) | |
| 3 | 1 | drop | protein | 20 (mg/ml) | |
| 4 | 1 | reservoir | sodium acetate | 25 (mM) | |
| 5 | 1 | reservoir | ammonium nitrate | 5 (M) |
