1REC
THREE-DIMENSIONAL STRUCTURE OF RECOVERIN, A CALCIUM SENSOR IN VISION
Experimental procedure
| Spacegroup name | I 4 |
| Unit cell lengths | 85.100, 85.100, 60.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 * - 1.900 |
| R-factor | 0.191 |
| Rwork | 0.191 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.460 |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 1.900 * |
| Rmerge | 0.078 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10-17 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 50 (mM) | |
| 3 | 1 | drop | dithiothreitol | 1 (mM) | |
| 4 | 1 | drop | 1 (mM) | ||
| 5 | 1 | drop | 1 (mM) | ||
| 6 | 1 | drop | sodium azide | 0.05 (%) | |
| 7 | 1 | drop | ammonium sulphate | 35-45 (%sat) | |
| 8 | 1 | reservoir | Tris-HCl | 50 (mM) | |
| 9 | 1 | reservoir | ammonium sulfate | 70-90 (%sat) |
