1RDV

RUBREDOXIN FROM DESULFOVIBRIO VULGARIS MIYAZAKI F, TRIGONAL CRYSTAL FORM

Experimental procedure

Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-6A
Synchrotron site	Photon Factory
Beamline	BL-6A
Temperature [K]	288
Detector technology	IMAGE PLATE
Detector	FUJI
Spacegroup name	P 32 2 1
Unit cell lengths	43.700, 43.700, 50.700
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.000
R-factor	0.208
Rwork	0.208
R-free	0.27200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7rxn
RMSD bond length	0.011
RMSD bond angle	1.691 *
Data reduction software	WEIS
Data scaling software	FSCALE
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.000
Rmerge	0.089
Total number of observations	10630 *
Number of reflections	2541 *
Completeness [%]	53.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Microdialysis *	7 *	10 *	Higuchi, Y., (1998) Protein Pept.Lett., 5, 175. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	10 (mg/ml)
2	1	1	Tris-HCl	50 (mM)
3	1	1	ammonium sulfate	60-70 (%sat)
4	1	2	ammonium sulfate	75-80 (%sat)