1RDS

CRYSTAL STRUCTURE OF RIBONUCLEASE MS (AS RIBONUCLEASE T1 HOMOLOGUE) COMPLEXED WITH A GUANYLYL-3',5'-CYTIDINE ANALOGUE

Experimental procedure

Spacegroup name	P 21 21 21
Unit cell lengths	46.520, 60.570, 34.950
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 1.800
R-factor	0.204
RMSD bond length	0.016
RMSD bond angle	0.040
Refinement software	PROLSQ

Data quality characteristics

	Overall
Low resolution limit [Å]	6.000 *
High resolution limit [Å]	1.800 *
Rmerge	0.065 *
Total number of observations	23821 *
Number of reflections	7554 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6 *	20 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	2.0 (%)
2	1	drop	3'-GMP	0.29 (%)
3	1	drop	MPD	27 (%(v/v))
4	1	reservoir	MPD	65 (%(v/v))
5	1	drop	acetate	35 (mM)