1RDR
POLIOVIRUS 3D POLYMERASE
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 243 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-03 |
| Detector | RIGAKU |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 88.131, 88.131, 158.395 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.000 - 2.400 |
| R-factor | 0.244 |
| Rwork | 0.244 |
| R-free | 0.27400 |
| Structure solution method | HEAVY ATOM ISOMORPHOUS REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 21.300 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS (0.3) |
| Refinement software | CNS (0.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.580 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.087 | 1.000 |
| Number of reflections | 27135 | |
| <I/σ(I)> | 9.4 | 0.7 |
| Completeness [%] | 95.0 | 80 |
| Redundancy | 3.1 | 1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.6 | 15-22 * | pH 6.6 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 3.5 (mg/ml) | |
| 2 | 1 | drop | 0.6 (M) | ||
| 3 | 1 | drop | PIPES | 50 (mM) | |
| 4 | 1 | drop | glycerol | 3 (%) | |
| 5 | 1 | drop | dithiothreitol | 10 (mM) | |
| 6 | 1 | drop | EDTA | 0.2 (mM) | |
| 7 | 1 | reservoir | 1.2 (M) |
