1RDI
MANNOSE-BINDING PROTEIN, SUBTILISIN DIGEST FRAGMENT COMPLEX WITH ALPHA-METHYL-L-FUCOPYRANOSIDE
Experimental procedure
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-04-08 |
| Detector | RIGAKU RAXIS IIC |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 60.700, 75.100, 57.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.800 |
| R-factor | 0.196 |
| Rwork | 0.196 |
| R-free | 0.24400 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 19.900 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 10.000 |
| High resolution limit [Å] | 1.800 |
| Rmerge | 0.054 |
| Number of reflections | 24379 |
| Completeness [%] | 97.8 |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.4 | 22 * | pH 7.4, 20% MPD USED AS A CRYOPROTECTANT |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 12-20 (mg/ml) | |
| 10 | 1 | reservoir | 2 (mM) | ||
| 11 | 1 | reservoir | 12 (mM) | ||
| 2 | 1 | drop | Tris-HCl | 110 (mM) | |
| 3 | 1 | drop | 24 (mM) | ||
| 4 | 1 | drop | PEG8000 | 11-14 (%(w/v)) | |
| 5 | 1 | drop | 100 (mM) | ||
| 6 | 1 | drop | 2 (mM) | ||
| 7 | 1 | reservoir | PEG8000 | 11-14 (%(w/v)) | |
| 8 | 1 | reservoir | Tris-HCl | 100 (mM) | |
| 9 | 1 | reservoir | 100 (mM) |
